Laboratory training for students in Pecs

Methodical course on laboratory immunetechniques in molecular biology research was organized by the University of Pécs, Medical School, Clinical Centre, Department of Immunology and Biotechnology between 11 – 15 December 2017. (Course code: OPEL_B-139_NEP1) Selected topics were organized in work packages. Theoretical lectures held by professionals of the Department introduced each work package and followed in own-hand laboratory trainings assisted by experts of the Department. The examination was performed in written test form. The accomplishment of course was equal to 6 ECTS credits.

Laboratory immunetechniques in molecular biology research
PhD course schedule
Course code: OPEL_B-139_NEP1
December 11-15, 2017.

December 11, Monday
8.30-10.00
Introduction.
Monoclonal and polyclonal antibodies.
Immunization, hybridoma technique.
Antibody purification and storage.
Antibody labelling for practical applications.
Lecturer: Prof. Dr. Péter Németh

10.30-12.00
Immunocytochemistry, immunohistochemistry, fluorescent microscopy, confocal microscopy.
Lecturer: Prof. Dr. Péter Balogh

14.00-16.00
Immunohistochemistry practice.
Laboratory practice: Prof. Dr. Péter Balogh

December 12, Tuesday
8.30-10.00
Haemagglutination, Coombs-test.
Nephelometry, turbidimetry.
ELISA, RIA.
Routine diagnostics, automatization.
Lecturer: Prof. Dr. Tímea Berki

10.30-12.00
Immunodiffusion, immunoelectrophoresis.
Dot blot, Westen blot.
Immunoprecipitation.
Lecturer: Dr. Péter Engelmann

14.00-16.00
ELISA routine diagnostic practice.
Laboratory practice: Zsuzsa Csizmadia

December 13, Wednesday
10.30-12.00
Molecular DNA techniques and their immunologic applications I.
Phage display and its applications in immunology.
Lecturer: Dr. Ferenc Boldizsár

14.00-16.00
Molecular biology practice.
Laboratory practice: Dr. Erzsébet Gajdócsi

December 14, Thursday
8.30-10.00
Significance of cell surface CD markers. Flow cytometry.
Cell surface and intracellular staining for flow cytometry.
Lecturer: Dr. Péter Engelmann

10.30-12.00
New possibilities in flow cytometry.
CBA technique.
Lecturer: Dr. Ferenc Boldizsár

14.00-16.00
Flow cytometry practice.
Laboratory practice: Réka Kugyelka

December 15, Friday
8.30-10.00
Molecular DNA techniques and their immunologic applications II.
Lentiviral vectors.
Lecturer: Dr. Ferenc Boldizsár

10.00-12.00
Consultation

14.00-16.00
Exam